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Experiments​​

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22 Mandatory Biology Investigations
 

  1. Conduct a qualitative test for: starch, fat, a reducing sugar, a protein.

  2. Identify any 5 fauna and any 5 flora using simple keys. Identify a variety of habitats within the selected ecosystem.

  3. Identify and use various apparatus required for collection methods in an ecological study.

  4. Conduct a quantitative study of plants and animals of a sample area of the selected ecosystem. Transfer results to tables, diagrams, graphs, histograms or any relevant mode.

  5. Investigate any 3 abiotic factors present in the selected ecosystem. Relate the results to the choice of habitat selected by each organism identified in this study.

  6. Be familiar with and use the light microscope.

  7. Prepare and examine one animal cell and one plant cell – unstained and stained – using the light microscope (X100, X400)

  8. Investigate the effect of pH on the rate of one of the following: amylase, pepsin or catalase activity.

  9. Investigate the effect of temperature on the rate of one of the following: amylase, pepsin or catalase activity.

  10. Investigate the effect of heat denaturation on the activity of one enzyme.

  11. Prepare one enzyme immobilisation and examine its application.

  12. Investigate the influence of light intensity or carbon dioxide on the rate of photosynthesis.

  13. Prepare and show the production of alcohol by yeast.

  14. Conduct any activity to demonstrate osmosis.

  15. Isolate DNA from plant tissue.

  16. Investigate the growth of leaf yeast using agar plates and controls.

  17. Prepare and examine microscopically the transverse section of a dicotyledonous stem (X100, X400)

  18. Dissect, display and identify an ox or sheep’s heart.

  19. Investigate the effect of exercise on the breathing rate or pulse rate of a human.

  20. Investigate the effect of IAA growth regulator on plant tissue.

  21. Investigate the effect of water, oxygen and temperature on germination.

  22. Use starch agar or skimmed milk plates to show digestive activity during germination.

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 2025-2026 SECTION B:


*There are 22 experiments on the course, if you combine these experiments with studying Unit One that equates to 45% of your exam.*
 
Questions 8 – 10
​Each of the three questions will be aligned with a specific experiment section:

Q8: One question from these 8 experiments

  1. To test for starch, to test for lipids, to test for proteins, to test for reducing sugars.

  2. To use simple keys to identify five fauna and five flora.

  3. To use different apparatus to collect plants and animals.

  4. To carry out a quantitative survey of plants and a quantitative survey of animals.

  5. To investigate three abiotic factors.

  6. To be familiar with and to use a light microscope.

  7. To prepare a plant cell and an animal cell and examine using a light microscope.

  8. To isolate DNA from a plant tissue.


Q9: One question from these 7 experiments

  1.  To investigate the effect of pH on enzyme activity.

  2. To investigate the effect of temperature on enzyme activity.

  3. To investigate the effect of heat denaturation on enzyme activity. 

  4. To prepare an enzyme immobilisation and examine its application.

  5. To investigate the influence of light intensity or carbon dioxide on the rate of photosynthesis. 

  6. To prepare and show the production of alcohol using yeast.

  7. To demonstrate osmosis. 


Q10: One question from these 7 experiments

  1. To investigate the growth of leaf yeast using agar plates.

  2. To prepare and examine a transverse section (TS) of a dicot stem.

  3. To dissect and identify the parts of a heart.

  4. To investigate the effect of exercise on the pulse rate or on the breathing rate.

  5. To investigate the effect of IAA growth regulator on plant tissue.

  6. To investigate the effect of water, oxygen and temperature on germination.

  7. To use starch agar to show the digestive activity during germination.​

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